Phosphinothricin n-acetyltransferase
WebNov 27, 2024 · In the 1980s, the bialaphos resistance ( BAR) gene and its closely related homologue the phosphinothricin acetyltransferase ( PAT) gene were isolated from Streptomyces hygroscopicus and... WebMar 20, 2006 · Similarly, bar and pat genes isolated from Streptomyces hygroscopicus and S. viridochromogenes, respectively, have been inserted into plants to encode phosphinothricin N -acetyltransferase to detoxify glufosinate. Glufosinate-tolerant crops have been commercialized using one of these two transgenes. Download to read the full …
Phosphinothricin n-acetyltransferase
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Phosphinothricin acetyltransferase (EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase. This enzyme catalyses the following chemical reaction acetyl-CoA + phosphinothricin CoA + N-acetylphosphinothricin
WebBioCrick BioTech 88# Keyuan road,Hi-Tech Zone Chengdu, Sichuan 610042, PRC Email: [email protected] Tel:86-28-8543-3893 WebJun 1, 2014 · Phosphinothricin N-acetyltransferase (PAT) detoxifies the nonselective herbicide phosphinothricin (PPT, the ammonium salt of which is known as glufosinate), which is the active ingredient in ...
WebThe phosphinothricin acetyltransferase (PAT) proteins, which are encoded by the bar coding sequence from Streptomyces hygroscopicus or the pat coding sequence from Streptomyces viridochromogenes, are present in glufosinate-ammonium-tolerant plant varieties of various crops such as corn, cotton, rice, oilseed rape, and soybean. WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N -acetyltransferase ( pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells …
WebGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of Streptomyces soil bacteria. Glufosinate is a non-selective, contact herbicide, with some systemic action. Plants may also metabolize bialaphos and phosalacine, other naturally occurring …
WebPhosphinothricin N-acetyltransferase (PAT) detoxifies the nonselective herbicide phosphinothricin (PPT, the ammonium salt of which is known as glufosinate), which is … orange theory jensen beachWebl -Phosphinothricin ( l -Pt)-resistant plants were constructed by introducing a modified phosphinothricin- N -acetyl-transferase gene ( pat) via Agrobacterium -mediated gene … iphone xr reboot loopWebResistance to bialaphos, a non-selective herbicide, was intro duced into cotton through genetic engineering. A gene encoding phosphinothric in acetyltransferase (bar) from Streptomyces hygroscopicus was inserted into elite varieties of cotton through particle bombardment. Based on the marker gene, β-glucuronidase (gus) expression, a total of 18 … iphone xr red walmartWebPhosphinothricin N-acetyltransferase (PAT) detoxifies the nonselective herbicide phosphinothricin (PPT, the ammonium salt of which is known as glufosinate), which is the active ingredient in... iphone xr red 64gb unlockedWebphosphinothricin N-acetyltransferase (PAT) enzyme: eliminates herbicidal activity of glufosinate (phosphinothricin) herbicides by acetylation . Regulatory Approvals: Country, Year and Type of Approval. Country: Food direct use or processing: Feed direct use or processing: Cultivation iphone xr refurbished irelandWebReconstitution. It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH 2 O. Storage & Stability. Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping. iphone xr refurbished backmarketWebMay 26, 2024 · PAT protein overexpression and purification. PAT (phosphinothricin acetyltransferase) expression condition were varied to obtain high yield, and transformed E. coli was examined in various condition such as temperature, IPTG concentration and culturing time. IPTG concentration of 0, 0.5 and 1 mM concentration were used at 18 °C, … orange theory jersey city nj