Ovcar3 cisplatin resistance

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WebJul 17, 2024 · NIH-OVCAR3 Cells Are Resistant to ATR Inhibitor VE-821, but Sensitive to Carboplatin and the PARP Inhibitor, Rucaparib One of the key roles of ATR is signalling … WebOct 31, 2013 · To identify mechanisms of resistance, we derived multiple, independent resistant sublines of OVCAR-3 to CDK2 inhibitors. Resistant cells were extensively characterized by gene expression and copy number analysis, fluorescence-activated cell sorting profiling and conventional karyotyping.

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WebDec 29, 2024 · The expression of kallistatin was decreased in cisplatin-resistant A2780/DDP cells compared to A2780 cells (Fig. 5 a). Correspondingly, there was a concentration-dependent decrease in kallistatin expression in A2780 and OVCAR3 cells that, which were exposed to cisplatin at 0, 2, 4, and 8 μg/ml for 48 h. WebJun 8, 2024 · Cisplatin-resistant capability of OVCAR3/DDP cells and the parental cells was assessed using a CCK-8 assay at various concentrations of cisplatin. As presented in Fig. 1A, OVCAR3 cell viability was gradually decreased and almost completely inhibited following treatment with 7 µmol/l cisplatin. is a ocean wave transverse or longitudinal

Long non-coding RNA H19 mediates ovarian cancer cell …

WebThe highest PCSK9 expression among OC cell lines was identified in OVCAR3 cell lysates, whereas the cisplatin-resistant OVCAR3cis cell lysates showed lower PCSK9 levels. This difference, however, was not observed in the endometrioid … WebMicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line Oncol Lett. 2024 Aug;14 (2):1780-1786. doi: 10.3892/ol.2024.6348. Epub 2024 Jun 8. Authors WebMoreover, 1G8 significantly improved the cisplatin response of OVCAR3 cells. Our data suggest that secretory HE4 played a novel pro-survival autocrine role and was a target of the anti-HE4 nanobody to improve the therapeutic effects of cisplatin-based chemotherapy. is a ocean wave mechanical or electromagnetic

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Hsa-miR-105-1 Regulates Cisplatin-Resistance in Ovarian

WebJun 1, 2014 · Accumulating evidence suggests that autophagy plays an important role in chemoresistance (24, 25), yet, its involvement in cisplatin resistance in ovarian cancer … WebMethods: OVCAR3 cancer cells were treated with cisplatin, Ly 294002 (LY), and cisplatin+Ly to investigate the cytotoxicity effect of the mentioned groups via MTT … omaha burgers thawing instructionsWebJul 1, 2024 · Finally, the cisplatin-resistant OVCAR3/DDP cell line was successfully induced when the cells survived in a medium containing 10 μmol/L cisplatin for 2 … is ao chan can\\u0027t study good

"WebApr 30, 2024 · OVCAR3 cells were treated with cisplatin, melatonin, cisplatin + melatonin, and siRNA Akt. Reactive oxygen species levels were assessed. The expression of the proteins was determined by... " - Ovcar3 cisplatin resistance

Ovcar3 cisplatin resistance

MicroRNA-20a contributes to cisplatin-resistance and …

WebJan 4, 2024 · For women with ovarian cancer, a staggering 70% will become resistant to the front-line therapy, cisplatin. Although many mechanisms of cisplatin resistance have been proposed, the key mechanisms of such resistance remain elusive. WebEmergence of cisplatin-resistant cells from the OVCAR-3 ovarian carcinoma cell line with p53 mutations, altered tumorigenicity, and increased apoptotic sensitivity to p53 gene …

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WebFeb 20, 2015 · Ovarian cancer cells have differing levels of native resistance to cisplatin. Human OC cell lines SKOV3, OVCAR3, OV-90, 3AO and SKOV3 DDP were treated with increasing concentrations of cisplatin (0–500 umol/L) for 72 h. The viabilities indicated on the y axis were determined by MTT assays and normalized to control. WebApr 11, 2014 · OVCAR-3 is a cisplatin-resistant ovarian cell line that is widely used as an in vitro model for platinum resistance in ovarian cancer [68, 69]. Interestingly, OVCAR-3 is also resistant to...

WebThe effects of cisplatin alone or in combination with staurosporine on (B) HCT-116 (top graph) and MCF-7 (lower graph), and (C) OVCAR3 (top graph) and OVCAR4 (lower graph) cell proliferation were ...

WebMay 17, 2012 · Carboplatin and cisplatin, alone or in combination with paclitaxel, have similar efficacies against ovarian cancer (OVCA) yet exhibit different toxicity profiles. ... (CAOV3, OV90, OVCAR3, SKOV3 ... WebDec 12, 2024 · The chemotherapeutic drug cisplatin, which targets DNA, serves as one of the main staples in cancer treatment. Yet, the therapeutic application of cisplatin is limited by two major challenges: the occurrence of reversible and irreversible side effects due to non-specific toxicity, and the intrinsic or developing resistance of tumor cells toward …

WebThe effects of miR-519d on ovarian cancer cell proliferation and cisplatin chemosensitivity were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, flow cytometry, and Western blotting assay. A luciferase reporter assay was performed to validate the miR-519d binding sites on the 3' untranslated region of X-linked ...

WebConclusion: In SKOV3 and OVCAR3 epithelial ovarian cancer cell lines, the correlation between HDAC overexpression and cisplatin resistance was confirmed. However, the specific HDAC isoform associated with resistance to cisplatin varied depending on the ovarian cancer cell line. is a ocean saltwater or freshwaterWebMay 17, 2024 · To confirm the effectiveness of MOD, an additional four ovarian cancer cells were evaluated; CAOV3, TOV-112D as cisplatin-sensitive ovarian cancer cells (CSC) and SKOV3, OVCAR3 as cisplatin-resistant ovarian cancer cells (CRC) (Figure 2D). TOV-112D viability was reduced in a dose-dependent manner from 40 μg/mL, while CAOV3 did not … is a ocean freshwater or saltwaterWebApr 23, 2024 · The objective of this study was to further evaluate role of c-MYB in ovarian cancer’s cisplatin resistance. To elucidate the underlying mechanism of cisplatin … is a odor a physical or chemical propertyWebMicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line MicroRNA-20a contributes to cisplatin-resistance and migration of … is a odds ratio of 2.5 goodThe NIH:OVCAR-3 is a cisplatin refractory cell line established from malignant ascites of a patient with progressive adenocarcinoma of the ovary after combination chemotherapy with cyclophosphamide, Adriamycin, and cisplatin [ 1 ]. Thus, OVCAR3 serves as a model cell line for drug resistance in ovarian cancer. is aoeah safeWebwere treated in the same manner but without cisplatin. The established cisplatin-resistant SW626 and OVcar3 cells were designated as SW626/DDP and OVcar3/DDP, respectively. To eliminate the inﬂuence of residual cisplatin in culture medium, SW626/DDP and OVcar3/DDP cells were cultured in cisplatin-free DMEM supplementary medium for 2 … is a ocean bigger than a seaWebJun 8, 2024 · Cisplatin-resistant capability of OVCAR3/DDP cells and the parental cells was assessed using a CCK-8 assay at various concentrations of cisplatin. As presented … is a octopus a fish