His bind resin
WebbHis-tagging is the most widespread and versatile strategy used to purify recombinant proteins for biochemical and structural studies. Recombinant DNA methods are first … http://wolfson.huji.ac.il/purification/PDF/Protein_Refolding/NOVAGEN_NiNTA_purification_resins.pdf
His bind resin
Did you know?
WebbHis-tagged proteins bind best to Ni-agarose resin if you are using a slightly basic pH (7.5-8). If the pH gets more acidic, the Histidine residues will be protonated and thus are not... WebbThermo Scientific HisPur Cobalt Resin is a tetradentate chelating agarose resin charged with divalent cobalt (Co 2+ ). The resin provides a high degree of purity and may …
WebbDescription; Overview: Ni-NTA His•Bind® Resin is a high-performance Ni 2+-charged agarose used for rapid one-step purification of proteins containing a His•Tag® sequence by metal chelation chromatography.NTA chemistry minimizes metal leaching during purification and is compatible with up to 10 mM β-mercaptoethanol or 1 mM … WebbHis•Bind ® Purification Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA Brochures User Protocol …
WebbHisタグ融合タンパク質のアフィニティー精製は、タンパク質生物学研究における金属キレート支持体のアプリケーションの中でも最も一般的な方法です。 NTAキレート化学で固定化されたニッケルやコバルトなどの金属は、このアプリケーションに最適な系です（次項をご参照ください）。 さらに、Hisタグタンパク質の小スケール精製（96ウェル … WebbHis•Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods. Packaging 10, 100, 50 ml in Plastic ampoule Warning Toxicity: Standard Handling (A) Legal Information
WebbBead size: 45 to 165μM Binding capacity: ≥ 40mg protein per ml bed volume of resin. The binding capacity of the resin to various types of proteins may vary according to the protein characteristics such as the size of the protein. cOmplete His-Tag Purification Columns bind with a high specificity to the polyhistidine-tagged protein.
WebbI-NTA HIS BIND RESIN 蛋白纯化Ni柱 收藏 价格 ¥1680 品牌 novagen 货号 70666-3 产地 美国 技术资料 查看技术资料 丁香通用户服务中心 查看详情 在线咨询 其他70666 novagen NI-NTA...商家产品推荐 查看更多> 12 个月用药助手专业版会员-7.0及以上软件版本可用（自动发货） ¥990 丁香园纪念小药盒 ¥150 OCTADECANEDIOIC ACID MONO-TERT … flats for sale w13WebbHisPur Ni-NTA Resin and HisPur Cobalt Resin are alternative forms of IMAC, the former using nickel and the latter using cobalt as the chelated metal ion responsible for His-tag binding. Ni-NTA resins have been the most common IMAC resin choice for 6xHis-tag protein purifications because of the four metal-binding sites on the chelate, which … check the status of my license illinoisWebbGeneral description. Ni-NTA His•Bind Resin is a high-performance Ni 2+ -charged agarose used for rapid one-step purification of proteins containing a His•Tag sequence … check the status of my insurance claim geicoWebbHis•Bind ® Resin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. CoA Brochures User Protocol Citations 69670 View Pricing & Availability Please request Quote Recommended Products Overview Supporting Documentation Related Product & Applications Overview Description check the status of my irs refundWebbHis•Bind® metal chelation chromatography. His•Tag proteins can be purified under a wide range of either native or denaturing conditions. The His•Bind affinity resin has a very high capacity for target proteins; up to 20mg of fusion protein can be bound on a single 2.5ml column. The method is based 6 check the status of my housing applicationWebbNi-NTA His•Bind Resins use nitriloacetic acid (NTA) as the chelator, which has four sites available for interaction with metal ions. NTA chemistry minimizes leaching of the metal … flats for sale tyne and wearWebbHis•Bind® Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale … check the status of my license