Flag-tag mouse monoclonal antibody

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August undefined, 2024

WebThe FLAG™ Tag (peptide sequence DYKDDDK) is a short, ... The membrane was probed with DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor 488 (Cat. No. MA1-142-A488) at a dilution of 1:500 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging … WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence.

DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor™ 488

WebConfocal image showing positive staining for FLAG® on HEK-293T cells transfected with DDDDK-tagged PFKFB3 expression vector. Mouse … WebDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) DDDDK tag Monoclonal Antibody for ELISA. Host / Isotype. Mouse / IgG2b. Reactivity. human, mouse, rat . ... Monoclonal ANTI-FLAG? M2 … cups into grams flour

Flag Tag Monoclonal Antibody - Cusabio

WebNeuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells. We established monoclonal mouse anti-mouse neuritin antibodies by immunizing knock-out mice with two different neuritin-derived … WebKey features and details PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt, Sandwich ELISA Reacts with: Species independent Conjugation: PE. Ex: 488nm, Em: 575nm Isotype: IgG1 Get better batch-to-batch reproducibility with a recombinant antibody WebMouse monoclonal [F-tag-01] antibody to DDDDK Tag. Applications: ICC, WB: Reactivity: Species independent: Conjugate: Unconjugated: ... Anti-FLAG Tag Antibody [FG4R] … cups into grams cereal

DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor™ 488

DYKDDDDK Tag Monoclonal Antibody (L5) (MA1-142)

WebMonoclonal ANTI-FLAG ® M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells. … WebThe ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody … cups into tablespoons ukWebHost Species Mouse; Clonality Monoclonal; Purification Affinity purification using immunogen. Applications WB,IF IP; Species Reactivity Hu Ms Rt; Specificity The Flag … easy crab cake recipe with stuffing

"WebSpecificity: Flag-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal Flag-tagged proteins. Immunogen: A synthesized peptide. Western blot analysis of Flag-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with Flag-tagged protein lysate. β-Actin Rabbit Polyclonal Antibody " - Flag-tag mouse monoclonal antibody

Flag-tag mouse monoclonal antibody

DDDDK tag Monoclonal antibody (Binds to FLAG® tag …

WebThe Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides. Packaging 0.2, 1, 1 mg Application WebHA-tag Rabbit Monoclonal Antibody staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. ... HA Tag Mouse Monoclonal Antibody. AG8050. 50μl. Flag Tag Rabbit …

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WebMouse Antibody Monoclonal. Introduction. A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. … WebThe DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this …

WebMonoclonal Antibody for studying Flag-Tag. Cited in 396 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse … http://www.abpbiosciences.com/product/flag-tag/

WebWestern blot was performed using HRP-conjugated Anti-DYKDDDDK Mouse Monoclonal Antibody (Product # MA1-91878-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), HA-H3-FLAG, 40 µg (Lane 3), HA-H3-FLAG, 20 µg (Lane 4), HA … WebFlag Tag Monoclonal Antibody Datasheet Product Details Citations Customer Reviews and Q&A Product Details Citations Stomatin modulates adipogenesis through the ERK pathway and regulates fatty acid uptake and lipid droplet growth SC Wu,Nature communications,2024

WebJan 21, 2024 · For the primary antibody reaction, DYKDDDDK Tag (D6W5B) Rabbit monoclonal antibody (1:1000, 14793; Cell Signal Technology, Beverly, MA, USA) and anti-C23 (NCL) mouse monoclonal (1:500, sc-55486; Santa Cruz Biotechnology, Dallas, TX, USA) were used in the antibody solution, and the mixture was allowed to react with the …

WebThe MA1-142 antibody (clone L5) has been successfully used as a detection antibody in a direct ELISA with FLAG-tagged protein-coated plates. Similar Epitope as the FLAG tag … cups inventoryWebMouse monoclonal [F-tag-01] antibody to DDDDK Tag. Applications: ICC, WB: Reactivity: Species independent: Conjugate: Unconjugated: ... Anti-FLAG Tag Antibody [FG4R] (A85282) 10µg - 200µg / £90 – £280 1 Citation. Description: Mouse monoclonal (FG4R) antibody to DYKDDDDK Tag. Applications: easy crab cake dipping sauce recipeWebMBP Tag Mouse Monoclonal Antibody (HRP Conjugated) The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Our anti-MBP monoclonal antibody provides a simple solution to detect the expression of protein ... easy crab cake recipe food networkWebDYKDDDDK Tag Monoclonal Antibody ... -Mouse Monoclonal Antibody Acquista DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin, Invitrogen™ su. Italiano … easy crab cake casseroleWebKey features and details. PerCP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt. Reacts with: Species independent. Conjugation: PerCP. Ex: 482nm, Em: 675nm. Isotype: IgG1. cups inyectologiaWebThe antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG ® antibodies, fused to either the amino-terminus or carboxy-terminus of the target protein. Species … cups ipp everywhere cups is not installed